Applied Biosystems ABI PRISM 310 Genetic Analyzer Sequencer DNA POP4 POP6

Artikelnummer: 48

Kategorie: Thermocycler (PCR / QPCR)


2.999,00 €

inkl. 19% USt. ,



Manufacturer: Applied Biosystems

Model: ABI PRISM 310 Genetic Analyzer

S/N: 100000629

Description:

The 310 Genetic Analyzer is the standard in laboratory convenience. After  samples are placed in the sample tray, virtually all aspects of analysis are automated. There is no need to pour conventional polyacrylamide and  agarose slab gels or to load samples for electrophoresis. Instead, every function?from sample and gel loading to data collection and analysis?is  computer controlled. Equally important, preformulated ABI PRISM® polymers eliminate the guesswork involved with preparing gel  materials. The convenience of the system is further enhanced by ABI PRISM® reagent kits for DNA sequencing or PCR product analysis and by access  to Applied Biosystems worldwide technical support.

The 310 Genetic Analyzer simultaneously detects up to five different fluorescent  dyes in a single capillary providing multicolor analysis. This process is further simplified by fluorescent terminator sequencing chemistry,  which allows you to perform singletube sequencing reactions. For genotyping, multicolor detection increases throughput by allowing you to  multiplex several PCR samples labeled with multiple dyes. A labeled internal standard can be added to each run to provide exceptional fragment  sizing reproducibility.

Key Features:

- Easy Instrument Set-up
- Automated Gel Loading
- Automated Sample Loading
- ABI PRISM® Separation Polymers
- Multiple Dye Detection
- ABI PRISM® System Integration

Specifications:

DNA Sizing and Quantitation
Template Types: PCR fragments, Restriction fragments, cDNA
Fluorescent Dye-Labeling Strategies: 5' dye-labeled primers

DNA Sequencing
Template Types: Single-stranded DNA, Double-stranded DNA, PCR-generated DNA
Fluorescent Dye-Labeling Strategies: 5' dye-labeled primers, Dye-labeled  terminators (2'-, 3'-dideoxynucleoside triphosphates) for use with unlabeled  primers
Standard Primers: M13 reverse, -21 M13, T3, T7, SP6, SK, KS

Hardware/Electronics
Detection Unit: CCD camera detector monitors fluorescent wavelengths from 525 to  650 nm. Virtual filter sets are optimized for ABI PRISM® dye sets.
Optics: Excites dyes by the 488 and 514 nm lines of the 10 mW argon ion laser.
Electrophoresis Power Supply: Controls voltage from 100 V to 15 kV.
Gel Pump: Loads capillary with polymer; generates pressures up to 1,800 psi.
Sample Trays: Holds 48 or 96 sample tubes for unattended operation.
Capillary Heat Plate: Controls capillary temperature between ambient and 60° C.
Windows® Computer
Controls all system functions for DNA
sequencing and genotyping.
Dimenstions WxDxH: 71 x 66 x 87cm
Weight: 95kg
Mains: 200/240 VAC 50/60Hz

Items delivered:

- ABI Prism 310 Genetic Analyzer
- Power Cord
- Instruction Manual as PDF via E-Mail

The instrument is out of a german university lab and has been serviced  regularly under service contract. It was sorted out after last service.
We did no further testing with the system. It comes without our regular function  guarantee.

 

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