Manufacturer: Applied Biosystems
Model: ABI PRISM 310 Genetic Analyzer
The 310 Genetic Analyzer is the standard in laboratory convenience. After samples are placed in the sample tray, virtually all aspects of analysis are automated. There is no need to pour conventional polyacrylamide and agarose slab gels or to load samples for electrophoresis. Instead, every function?from sample and gel loading to data collection and analysis?is computer controlled. Equally important, preformulated ABI PRISM® polymers eliminate the guesswork involved with preparing gel materials. The convenience of the system is further enhanced by ABI PRISM® reagent kits for DNA sequencing or PCR product analysis and by access to Applied Biosystems worldwide technical support.
The 310 Genetic Analyzer simultaneously detects up to five different fluorescent dyes in a single capillary providing multicolor analysis. This process is further simplified by fluorescent terminator sequencing chemistry, which allows you to perform singletube sequencing reactions. For genotyping, multicolor detection increases throughput by allowing you to multiplex several PCR samples labeled with multiple dyes. A labeled internal standard can be added to each run to provide exceptional fragment sizing reproducibility.
- Easy Instrument Set-up
- Automated Gel Loading
- Automated Sample Loading
- ABI PRISM® Separation Polymers
- Multiple Dye Detection
- ABI PRISM® System Integration
DNA Sizing and Quantitation
Template Types: PCR fragments, Restriction fragments, cDNA
Fluorescent Dye-Labeling Strategies: 5' dye-labeled primers
Template Types: Single-stranded DNA, Double-stranded DNA, PCR-generated DNA
Fluorescent Dye-Labeling Strategies: 5' dye-labeled primers, Dye-labeled terminators (2'-, 3'-dideoxynucleoside triphosphates) for use with unlabeled primers
Standard Primers: M13 reverse, -21 M13, T3, T7, SP6, SK, KS
Detection Unit: CCD camera detector monitors fluorescent wavelengths from 525 to 650 nm. Virtual filter sets are optimized for ABI PRISM® dye sets.
Optics: Excites dyes by the 488 and 514 nm lines of the 10 mW argon ion laser.
Electrophoresis Power Supply: Controls voltage from 100 V to 15 kV.
Gel Pump: Loads capillary with polymer; generates pressures up to 1,800 psi.
Sample Trays: Holds 48 or 96 sample tubes for unattended operation.
Capillary Heat Plate: Controls capillary temperature between ambient and 60° C.
Controls all system functions for DNA
sequencing and genotyping.
Dimenstions WxDxH: 71 x 66 x 87cm
Mains: 200/240 VAC 50/60Hz
- ABI Prism 310 Genetic Analyzer
- Power Cord
- Instruction Manual as PDF via E-Mail
The instrument is out of a german university lab and has been serviced regularly under service contract. It was sorted out after last service.
We did no further testing with the system. It comes without our regular function guarantee.