Manufacturer: Applied Biosystems / Thermo Scientific
Model: StepOne Real-Time PCR System
S/N: 271001372 (2008)
The StepOne? Real-Time PCR System is a 48-well, low-throughput Real-Time PCR instrument perfect for both first-time and experienced users.
The StepOne? Real-Time PCR System can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Utilizing robust LED based 3-color optical recording, the StepOne? Real-Time PCR System is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications.
? Advanced software and instrumentation for performing a wide array of genomic assays.
? Sensitive LED-Based 3-Color Fluorescence Reading
? The StepOne? Real-Time PCR System utilizes a long-life LED-based optical system that can record fluorescence from FAM?/SYBR® Green, VIC®/JOE?, and ROX? dyes.
? This cost-effective, 3-color, 48-well system delivers precise, quantitative Real-Time PCR results and saves data from all filters in every run without depending on a
? computer or plate setup. It can discriminate between 2 populations of 5,000 and 10,000 template copies of a TaqMan® assay with 99.7% confidence.
Rapid and Simple Assay Set-Up
This remarkably simple Real-Time PCR system is designed with an LCD touchscreen and a user-friendly interface that brings the power of genetic studies to researchers new to Real-Time PCR (Fig 1). The intuitive software and protocol wizards help guide new users through their Real-Time PCR experiments. The StepOne? Real-Time PCR System also includes a quick-start setup so that you start a run immediately and enter plate information at a later time.
Compatible with Many Genomic Analysis Techniques
Perform a variety of standard and demanding genetic analysis research techniques on one instrument using the user friendly StepOne? Real-Time PCR System and software. The StepOne? System supports many Real-Time PCR applications, including the following:
? SNP Genotyping
? Gene Expression Analysis
? MicroRNA Expression
? Translocation Analysis
? Gene Detection
? Viral Load Analysis
The included StepOne? Software supports a variety of analysis methods, including the following:
? Standard Curve (absolute quantitation)
? Relative Standard Curve
? Comparative Ct (??Ct) (relative quantitation)
? Genotyping and Presence/Absence
? Melt Curve Analysis
? High Resolution Melting (additional software required)
Thermal Cycling System: Peltier-based system
Block Format: 48-well block
Compatible Consumables: 48-well plates with optical flat caps or adhesive cover; 8-tube (0.1 mL) strips with optical flat caps; Individual (0.1 mL) tubes with optical flat caps
Supported Volumes: 10?30 µl (Note: For standard curve experiments, 40 µl in standard mode is validated.)
Sample Ramp Rate: Fast mode: +/- 2.2 °C/sec; Standard mode: +/- 1.6°C/sec
Peak Block Ramp Rate: 4.6 °C/sec
Temperature Range: 4°C-100°C
Temperature Accuracy: +/-0.25°C (35°C to 95°C) of setpoint/display temperature, measured at 3 minutes after clock start.
Temperature Uniformity: +/-0.50°C, 30 seconds after clock start.
Melt Curve Resolution: As small as 0.1°C
Optical System: LED, emission filters, and photodiode
Calibrated Dyes at Installation: SYBR® Green I, FAM?, VIC®, JOE?, ROX? dyes
Passive Reference Dyes: ROX? or any calibrated dye. Option exists to select no passive reference.
Data Collection: Data collected in all 3 filters for all wells regardless of plate setup. Plate setup may be altered after run completes.
Fast cycling: Less than 40 minutes
Quantitative PCR Run Time: Standard cycling: Less than 2 hrs
Fast cycling: Less than 40 minutes
Touchscreen: LCD/6.5 in. Full VGA (640x480)/32K colors
Instrument Dimensions (WxDxH): 24.6 x 42.7 x 51.2 cm
Weight: 23.6 kg (52 lbs.)
- Applied Biosystems StepOne Real Time PCR System
- Control Laptop with preinstalled/preconfigured Software
- Connection Cables & Power Cord
The Real Time PCR System is in very good cosmetic condition and 100% functional tested.