Manufacturer: Leica Microsystems
Model: DMi6000B CS + TCS SP5 Confocal Microscope System
Laser: HeNe (543nm, 594nm, 633nm) / Argon (458nm, 476nm, 488nm, 496nm, 514nm) / Diode UV 405nm
Status: The extensive equipped system is completely reinstalled on a brand new computer with WIN10 and Leica LAS AF273, reinitialized and tested by Leica Service Engineer with Service report and Maintenance Batch on the device. The System is fully functional tested and ready to deliver good performance.
System Description:
Confocal microscope system with a broad range of scan speeds suited for high-resolution morphological imaging, as well as multispectral imaging.
- 350-900 nm spectral detection range with 5 nm spectral resolution and sub-micron spatial resolution
- Suitable for most fluorophores from DAPI to Cy5
- Measuring up to five dimensions, including x, y, z, time and spectrum
- Capable of multi-dimensional fluorescence qualitative and quantitative measurement, FRET, FRAP, FLIP, FLAP and other photobleaching-related applications
- Suitable for imaging fixed specimen (No environmental chamber).
Internal Detectors
- Spectral detection channels: PMT1, PMT2, PMT3
- Brightfield transmission channel: PMT Trans
Multi-dimension capability: xyz, xzy, xt, xyt, xzt, xyzt, xy, xz, xyt, xyz
Stage:
- Manual moving X, Y stage
- Motorized z stage (piezo-controlled) with 166.68 µm scanning range
Zoom range: 1x-32x
Pinhole size: 20 µm-600 µm
Contrast enhancement options: Brightfield, DIC and polarized
Software: Leica LAS AF, Version 2.7.3 on Windows 10, for image acquisition and processing
Scanners
Conventional scanner: 10-1400 Hz (2-3 frames/sec at 512 x 512 pixels)
Internal detectors
Reflection/fluorescence mode (spectral detection range 400-800 nm)
- Spectral detection channels: PMT1, PMT2, PMT3
- Additional PMT in transmission mode
Type Wavelenght (nm) Dyes and use
UV 405 DAPI, Hoechst
Argon 458, 476, 488, 496, 514 CyanFP, GFP, Alexa488, CY2, YFP
HeNe 543 Alexa 546, Alexa 555
HeNe 594
HeNe 633 Cy5, Alexa 647
- An acousto-optical tunable filter (AOTF) controls the intensity of the visible excitation laser light.
- An acousto-optical beam splitter (AOBS) is used to split excitation light and emission light instead of filter blocks.
Microscope
Leica DMI 6000B CS Trino inverted microscope with motorized XYZ-stage
Objectives:
- HCX PL FL L 40x/0.60 CORR PH2 0-2/C (
- HCX PL APO 63x/1.40-0.60 Oil. Lbd. BL
- HC PL FLUOTAR 10x/0.30 Ph1
- HCX PL APO 40x/1.25 Oil Ph3 CS
- HCX PL FL L 63x/0.70. CORR
- HI PLAN 20x/20X/0.40
Fluorescent Source
Leica Lamphouse 106z HG 100W light source with LEJ EBQ100 Mercury Burner Power Supply for fluorescence excitation.
Fluorescence Filters:
- A4
- L5
- Y5
- N2.1
- Analyzer
Condition:
The extensive equipped system is completely reinstalled on a brand new computer with WIN10 and Leica LAS AF273, reinitialized and tested by Leica Service Engineer with Service report and Maintenance Batch on the device. The System is fully functional tested and ready to deliver good performance.
Complete German Configuration Summary:
156504116 SuperZ Galvo Tisch Typ H
156504114 Einsatz rot Typ H
156504145 3 Plattenkreuztisch mot SP5 invers
15500325 Vorschaltgerät ebq 100 iso-l
15000906000001 PMT-Modul wassergekühlt SP5/SP8
155934681 Optical Outfit DMI 6000 CS w. ICT
11506239 HI PLAN 20x/20X/0.40
15506203 HCX PL FL L 40x/0.60 CORR PH2 0-2/C
15506192 HCX PL APO 63x/1.40-0.60 Oil. Lbd. BL
15506507 HC PL FLUOTAR 10x/0.30 Ph1
15506181 HCX PL APO 40x/1.25 Oil Ph3 CS
15506216 HCX PL FL L 63x/0.70. CORR
15521505 Lichtringset f. Kondensor S23/S28
156501100 Basismodul AOBS
156504750 Bedienkonsole programmierbar mit LCDs
156501120 konventionelles Scanningsystem SP5 II
156504500 X1 Port Emission Access
156502123 Imaging Laser ROGB für AOBS
156501201 Laser Port UV/405nm
156502300 Imaging Laser 405nm cw 50mW
156502352 AOTF UV/405
156502379 UV Optik 09
156504201 BF Detektor für DMI
156503122 Workstation Plus 2x LCD
15522023 Scanningtisch
15500138 HF-Höchstdruck-Lampe 12V 100W
156501330 SP Kanal 03
156501390 Konfokal Detektor IMG
15541537 C-Mount Adapter HC 0.63X
15513874 Filtersystem A4
15513880 Filtersystem L5
15513888 Filtersystem Y5
Filter N2.1
Analyzer
155933630 DMI 6000 BP CS Trino
156504100 SuperZ Galvo Tisch invers
15504111 Fl-Lamp Holder for DMI, straight