Manufacturer: Thermo Scientific
Model: Appliscan Multimode Plate Reader
P/N: 5230000
S/N: 2001-2080114
MFG date: 2008
Description:
Thermo Scientific Appliskan Multimode Microplate Reader is a filter-based multitechnology microplate reader for photometric, luminometric and fluorometric research applications. The Appliskan features an onboard shaker and an incubator. Up to two optional onboard dispensers are possible for easy reagent additions and exact follow-up of kinetic reactions. It can work with numerous plate formats from 6- to 384-well.
Appliskan is a true multimode microplate reader capable of measuring:
- Fluorescence intensity
- Luminescence (flash and glow)
- Absorbance (low UV to visible)
- Time-resolved fluorescence (TRF)
- Fluorescence polarization (FP)
- Dual-label measurements (such as BRET, FRET and TR-FRET)
The Appliskan’s functions are controlled by SkanIt Software, providing easy assay design, flexible data handling and analysis, convenient report formatting and giving you the power to maximize any application.
Features:
- Complete flexibility and multiple functions in one small footprint
- Excellent sensitivity – you can be sure of your measurement results
- Numerous plate formats
- Easy-to-use SkanIt Software – accelerates design, development and reporting of even the most complex assays through the intuitive steplist interface
- Precise dispensing – the Appliskan can be fitted with up to two dispensers enabling flash applications. Moreover, the dispensing efficiency minimizes reagent usage
Areas of application:
Cytotoxicity
- Luminometry using ATP
- Photometry using tetrazolium, e.g. MTT, MTS
- Fluorometry using resorufin
DNA analysis
- Fluorometric quantitation
- Photometric quantitation (260 nm / 280 nm)
- Luminometric human specific quantitation
Multiplexing
- Fluorometric FRET
- Luminometric dual reporter gene
- Dual-label Time-Resolved Fluorescence
Protein activity
- Fluorometric kinase assay
- Receptor binding with Fluorescence Polarization
- Second messengers with Time-Resolved Fluorescence
- Luminometric ion channel activity
Immunoassays
- Photometric ELISA
- Fluorometric FIA
- Time-Resolved Fluorometric TR-FIA
- Luminometric LIA
Specifications:
- Overall dimensions: ca. 38 cm (W) x 50 cm (D) x 34 cm (H)
- Weight: Instrument: 27 kg; Mains power supply box: 3.6 kg
- Mains power supply: 100 – 240 Vac, 50/60 Hz
- User interface: The instrument is under PC control and runs on SkanIt Software for Appliskan, which controls all the instrument functions and provides data reduction as well as reporting functions
- Computer interface: RS-232C
- Light source: Xenon flash lamp
- Detector: Photomultiplier tube’s for fluorometry and luminometry, and a photodiode for photometry
- Measurement types: Fluorescence intensity, time-resolved fluorescence, fluorescence polarization, absorbance and luminescence
- Wavelength selection: By filters: excitation filters (diam.12.5 mm), emission filters (diam. max. 25,4 mm) and absorbance filters (diam. 12.5 mm)
- Plate types: 6 to 384-well plates
- Fluorometry:
Excitation wavelength range: 200 – 1000 nm
Emission wavelength range: 360 – 820 nm
Sensitivity: Fluorescence intensity: < 2 fmol fluorescein/well, 384-well plate; Time-resolved fluorescence: < 20 amol Europium/well, 384-well plate Precision: Fluorescence polarization: < 10 mP 1 nM fluorescein, 96-well plate
Dynamic range: > 5 decades
Measurement time: Fluorescence intensity: 10 – 1000 ms; Time-resolved fluorescence : 5 – 10000 ms
- Photometry:
Wavelength range: 200 – 1000 nm
Linearity: 0 – 2.5 Abs (96-well plate) at 450 nm, ± 2%; 0 – 2 Abs (384-well plate) at 450 nm, ± 2%
Measurement range: 0 – 4 Abs
Absorbance resolution: 0.001 Abs
Measurement time: 20 – 1000 ms
- Luminometry:
Emission wavelength range: Standard mode: 360 – 820 nm; High-sensitive mode: 300-630 nm
Sensitivity: High-sensitive mode: < 10 amol ATP/well, 384-well plate; Standard mode: < 200 amol ATP/well, 384-well plate
Dynamic range: > 5 decades Measurement time: 10 – 10000 ms
- Incubator (measurement chamber):
Temperature range: From ambient + 4°C to 45°C
- Shaker:
Shaking method: Linear shaking
Shaking amplitude: 1 – 10 mm
Items delivered:
- Thermo Scientific Appliscan Multimode Plate Reader
- Power Supply (S/N: 2080115)
- PC (Tower, Monitor, Keyboard)
- 2x Spare Fuses 5A
- 2x Appliscan User Manuals (Appliscan & Software for Appliscan)
- SkanIt Software 2.3 (Research Edition CD)
- Power Cord
- Serial Cable
(For further accessories please see pictures.)
Condition:
This instrument is in excellent cosmetic condition. It is 100% functional tested and ready for your operations.